Mometasone furoate monohydrate, process for making same and pharmaceutical compositions

ABSTRACT

The invention relates to the novel compound mometasone furoate monohydrate, process for its preparation and pharmaceutical compositions containing said compound.

This application is a 371 of PCT/US91/06249 filed Sep. 6, 1991.

BACKGROUND OF THE INVENTION

The present invention relates to a novel composition of matter, 9α, 21-dichloro-16α-methyl-1,4-pregnadiene-11β,17α-diol-3,20-dione-17-(2'-furoate) monohydrate, also designated mometasone furoate monohydrate, process for its preparation, and pharmaceutical preparation thereof.

Mometasone furoate is known to be useful in the treatment of inflammatory conditions. The compound is prepared by procedures disclosed in U.S. Pat. No. 4,472,393, which patent is hereby incorporated by reference.

When aqueous pharmaceutical compositions, e.g. suspensions, containing anhydrous mometasone furoate were subjected to stability testing by rotating for four weeks at room temperature and 35° C., formation of a crystalline material which is different from the anhydrous mometasone furoate crystal was observed in suspension. Experiments were designed to determine the nature of the crystalline material. It was postulated that formulation of mometasone furoate compositions with the stable crystalline form would reduce the probability of crystal growth during long term storage of the suspension leading to a more stable product.

SUMMARY OF THE INVENTION

The present invention provides mometasone furoate monohydrate of formula I ##STR1## a process for preparing said compound by crystallization from a saturated aqueous water miscible organic solution. The present invention also provides aqueous stable pharmaceutical compositions of mometasone furoate monohydrate.

DESCRIPTION OF THE FIGURES

FIG. 1: Infrared spectrum of crystalline mometasone furoate monohydrate

FIG. 2: X-ray diffraction pattern of crystalline mometasone furoate monohydrate

DETAILED DESCRIPTION OF THE INVENTION

The composition of matter of the present invention, mometasone furoate monohydrate has the following characteristics.

Molecular formula C₂₇ H₃₀ Cl₂ O₆ H₂ O

Formula weight 539.46

Elemental Analysis (theory) C=60.11%, H=5.98%; Cl=13.16% (found) C=59.99%; H=5.56%; Cl=13.17%

Water Analysis (% H₂ O) (theory) 3.34% (found) 3.31, 3.47

The crystalline mometasone furoate monohydrate exhibits an x-ray crystallographic powder diffraction pattern having essentially the values as shown in Table I.

                  TABLE 1                                                          ______________________________________                                         Angle of      Spacing                                                          2θ      d        Relative Intensity                                      (degrees)     (Å)  |/|                                   ______________________________________                                          7.795        11.3324  100                                                     11.595        7.6256   6                                                       12.035        7.3478   3                                                       12.925        6.8437   11                                                      14.070        6.2893   22                                                      14.580        6.0704   5                                                       14.985        5.9072   12                                                      15.225        5.8146   33                                                      15.635        5.6631   96                                                      16.710        5.3011   15                                                      17.515        5.0592   14                                                      18.735        4.7324   12                                                      20.175        4.3978   13                                                      20.355        4.3593   6                                                       20.520        4.3246   4                                                       21.600        4.1108   5                                                       21.985        4.0396   22                                                      22.420        3.9622   8                                                       22.895        3.8811   7                                                       22.245        3.8234   14                                                      23.550        3.7746   13                                                      24.245        3.6680   4                                                       24.795        3.5878   11                                                      24.900        3.5729   5                                                       24.800        3.4503   5                                                       25.985        3.4262   3                                                       26.775        3.3268   84                                                      27.170        3.2794   10                                                      27.305        3.2635   9                                                       27.710        3.2167   5                                                       28.385        3.1417   7                                                       29.165        3.0594   1                                                       29.425        3.0330   2                                                       29.725        3.0030   2                                                       30.095        2.9670   7                                                       30.255        2.1516   3                                                       30.490        2.9294   10                                                      30.725        2.9075   6                                                       31.115        2.8720   3                                                       31.595        2.8294   47                                                      32.135        2.7831   6                                                       32.985        2.7133   7                                                       33.400        2.6805   2                                                       33.820        2.6482   2                                                       34.060        2.6301   8                                                       34.625        2.5885   4                                                       34.795        2.5762   2                                                       35.315        2.5394   1                                                       36.780        2.4416   21                                                      37.295        2.4090   2                                                       ______________________________________                                    

Single crystal data of mometasone furoate monohydrate exhibits the following values as shown in Table II.

                  TABLE II                                                         ______________________________________                                         Crystallographic Data.sup.a                                                    Crystal system triclinic                                                       Space group    P1(C.sup.1.sub.1)-No. 1                                         ______________________________________                                         a (Å)      8.481 (1)                                                       b (Å)      11.816 (2)                                                      c(Å)       7.323 (1)                                                       α(°)                                                                             95.00 (1)                                                       β(°)                                                                              110.66 (1)                                                      γ(°)                                                                             73.27 (1)                                                       V (Å.sup.3)                                                                               657.5 (3)                                                       D.sub.calcd. (g cm.sup.-3)                                                                    1.362.sup.                                                      ______________________________________                                    

a An Enraf-Nonius CAD-4 diffractometer (Cu-Kα radiation, incident-beam graphite monochromator) was used for all measurements, Intensity data were corrected for the usual Lorentz and polarization effects; an empirical absorption correction was also applied.

The crystal structure was solved by direct methods (RANTAN). Approximate non-hydrogen atom positions were derived from an E-map. Hydrogen atoms were located in a series of difference Fourier syntheses evaluated following several rounds of full-matrix least-squares adjustment of non-hydrogen atom positional and anisotropic temperature factor parameters. Hydrogen atom positional and isotropic thermal parameters were included as variables in the later least-squares iterations which also involved refinement of an extinction correction. Crystallographic calculations were performed on PDP11/44 and MicroVAX computers by use of the Enfra-Nonius Structure Determination Package (SDP). For all structure-factor calculations, neutral atom scattering factors and their anomalous dispersion corrections were taken from International Tables for X-Ray Crystallography, vol. IV, The Knynock Press, Birmingham, England, 1974.

Mometasone furoate monohydrate can be prepared by forming a saturated homogeneous solution of anhydrous mometasone furoate in a mixture of water and a water miscible organic solvent. The saturated solution is prepared by dissolving the mometasone furoate in a water miscible organic solvent at the temperature of about 85° C. Hot water, about 85° C., is added dropwise with agitation. After removing the solution from the steam bath, the reaction is stirred for about one hour and then allowed to stand undisturbed overnight while cooling to room temperature. The solution is stirred while adding additional water at room temperature and the solution becomes cloudy and a white precipitate forms. The reaction is allowed to stir for a time, the preciptitate collected by filtration and the product dried to constant weight.

Organic solvents that can be employed in the process of this invention must be miscible with water and one in which mometasone furoate is soluble. Examples of water miscible organic solvents include alcohols, such as, ethanol, isopropanol, and the like; ketones, such as acetone, and the like; ethers, such as dioxane, and the like; esters such as ethyl acetate, and the like. The preferred solvents are acetone and isopropanol.

In another aspect, the present invention provides pharmaceutical compositions comprising mometasone furoate monohydrate of formula I in an inert pharmaceutically acceptable carrier or diluent.

The pharmaceutical compositions according to the invention can be prepared by combining mometasone furoate monohydrate with any suitable inert pharmaceutical carrier or diluent and administered orally, parentally or topically in a variety of formulations.

Of particular interest are aqueous suspension compositions of mometasone furoate monohydrate, e.g. for nasal administration. The aqueous suspensions of the invention may contain from 0.1 to 10.0 mg of mometasone furoate monohydrate per gram of suspension.

The aqueous suspension compositions according to the present invention may contain, inter alia, auxiliaries and/or more of the excipients, such as: suspending agents, e.g. microcrystalline cellulose, sodium carboxymethylcellulose, hydroxypropyl-methyl cellulose; humectants, e.g. glycerin and propylene glycol; acids, bases or buffer substances for adjusting the pH, e.g. citric acid, sodium citrate, phosphoric acid, sodium phosphate e.g. citrate and phosphate buffers; surfactants, e.g. Polysorbate 80; and antimicrobial preservatives, e.g. benzalkonium chloride, phenylethyl alcohol and potassium sorbate.

The following examples illustrate the present invention and the best made of practicing the process of the invention. It will be apparent to those skilled in the art that modifications thereof may be practical without departing from the purpose and intent of this disclosure.

General Experimental

Infrared absorption spectra were taken as Nujol Mull on a Nicolet FT-infrared spectrometer Model No. 5DXB. X-ray crystallograph powder diffraction patterns were taken on a Philips X-ray diffractometer Model APD-3720 equipped with a radiation source: copper Kα. Decomposition temperatures were measured on a Dupont differential scanning calorimeter, Model No. 990.

Moisture content of the crystalline mometasone furoate monohydrate was determined by titration with Karl Fisher reagent.

EXAMPLE 1

Place 4.5 liters of ethyl alcohol into a suitable vessel equipped with an appropriate agitator and closure. Dissolve 27 g of mometasone furoate anhydrous powder into the ethanol with stirring. Filter the saturated solution and slowly add purified water about 1.5 liters, at a flow rate of approximately 50 ml/minute while stirring at moderate speed. When the solvent mixture reaches a ratio of 1:3 (water:ethanol), the addition of water is stopped and stirring of the reaction mixture is continued for approximately 2 hours to facilitate seeding. Resume addition of water, about 7.5 liters at a rate of approximately 50 ml/minute, until a ratio of 2:1 (water:ethanol) is achieved. Continue stirring to complete crystallization. The crystals are collected by filtration and dried in a vacuum desiccator at room temperature to afford 24.83 g of mometasone furoate monohydrate having an infrared spectrum and X-ray diffraction graph substantially the same as that in FIGS. 1 and 2.

EXAMPLE 2

Place 24.3 liters of 2-propanol into a suitable container. Dissolve 340 grams of anhydrous mometasone furoate in the 2-propanol by heating the mixture (steam bath) to 85° C. with stirring. After the furoate has dissolved, add dropwise with stirring over 15 minutes 1950 ml of hot (85° C.) water. The hot solution is removed from the steam bath and the solution is stirred for 1 hour. The solution is allowed to cool to room temperature overnight without stirring. The remainder of water, about 24 liters is added with stirring; the solution becomes cloudy and a white precipitate begins to form. The reaction is stirred for one hour, following addition of the water. The white precipitate is collected by filtration, washed with 2 liters of water and air dried overnight. The solid is dried in a draft oven at 50° C. to constant weight. Mometasone furoate monohydrate, 316.5 g, weight yield 90%, is obtained having an infrared spectrum and X-ray diffraction graph substantially the same as that in FIGS. 1 and 2.

EXAMPLE 3

An aqueous nasal suspension of mometasone furoate monohydrate is prepared from the following:

    ______________________________________                                                       Concentration                                                                             Representative Batch                                  Ingredients   mg/g       g/12 kg                                               ______________________________________                                         Mometasone furoate                                                                           0.5        6.0                                                   monohydrate                                                                    Avicel RC 591*                                                                               20.0       240.0                                                 Glycerin      21.0       252.0                                                 Citric Acid   2.0        24.0                                                  Sodium citrate                                                                               2.8        33.6                                                  Polysorbate 80**                                                                             0.1        1.2                                                   Benzalkonium chloride                                                                        0.2        2.4                                                   Phenylethyl alcohol                                                                          2.5        30.0                                                  Purified water q.s. ad                                                                          1.0 g     12.0 kg                                             ______________________________________                                          *Avicel RC591-is a trademark of FMC for a mixture of microcrystalline          cellulose and sodium carboxymethyl cellulose.                                  **Polysorbate 80 is a tradename for a mixture of an oleate ester of            sorbitol and its anhydride copolymerized with approximately 20 moles of        ethylene oxide for each mole of sorbitol and sorbitol anhydride.         

After dispersing the Avicel RC 591 in 6 kg of purified water, the glycerin is added thereto. The citric acid and sodium citrate is dissolved in 240 ml of water, said solution is added to the Avicel-glycerin dispersion with mixing. In a separate vessel, Polysorbate 80 is dissolved in approximately 400 ml of purified water with stirring. The mometasone furoate monohydrate is dispersed in the aqueous Polysorbate 80 solution and; said slurry is then added with stirring to the Avicel-glycerin citric acid mixture. After dissolving benzalkonuim chloride and phenylethyl alcohol in purified water, said solution is added to the suspension mixture with stirring. The suspension is brought to 12 kg with purified water with mixing. The final pH of the suspension is 4.5±0.5.

EXAMPLE 4

The following compositions were prepared without the suspending agent, Avicel RC-591 to prevent interference in X-ray diffraction studies:

    ______________________________________                                                         Concentration                                                                  mg/g                                                           Ingredients     4A        4B      4C                                           ______________________________________                                         Mometasone Furoate                                                                             0.5       0.5     0.5                                          Monohydrate Micronized                                                         Citric Acid Monohydrate                                                                        2.0       2.0     2.0                                          Sodium Citrate Dihydrate                                                                       2.8       --      2.8                                          Sodium Phosphate Dibasic                                                                       --        4.0     --                                           Polysorbate 80  0.1       0.1     0.1                                          Benzalkonium Chloride                                                                          0.2       0.2     0.2                                          Phenylethyl Alcohol                                                                            2.5       --      --                                           Potassium Sorbate                                                                              --        3.4     --                                           Propylene Glycol                                                                               --        --      100.0                                        Glycerin        21.0      21.0    21.0                                         Water Purified USP q.s. ad                                                                        1.0 g     1.0 g                                                                                  1.0 g                                     ______________________________________                                    

These compositions were prepared according to the procedure described in Example 3.

The three compositions 4A, 4B and 4C were rotated for five (5) days at 35° C. and a additional four (4) weeks at room temperature to assess crystal form stability. The crystals were isolated from the suspension and X-ray diffraction patterns determined. The results indicated that the crystals collected from each of the three compositions are in the form of mometasone furoate monohydrate.

EXAMPLE 5

The following compositions were prepared and tested to determine thermal stability of said compositions.

    ______________________________________                                                         Concentration                                                                  mg/g                                                           Ingredients     4A        4B      4C                                           ______________________________________                                         Mometasone Furoate                                                                             0.5       0.5     0.5                                          Monohydrate Micronized                                                         Citric Acid Monohydrate                                                                        2.0       2.0     2.0                                          Sodium Citrate Dihydrate                                                                       2.8       --      2.8                                          Sodium Phosphate Dibasic                                                                       --        4.0     --                                           Polysorbate 80  0.1       0.1     0.1                                          Benzalkonium Chloride                                                                          0.2       0.2     0.2                                          Phenylethyl Alcohol                                                                            --        2.5     --                                           Potassium Sorbate                                                                              --        --      3.4                                          Propylene Glycol                                                                               100.0     --      --                                           Glycerin        21.0      21.0    21.0                                         Avicel RC-591   20.0      20.0    20.0                                         Water Purified USP q.s. ad                                                                        1.0 g     1.0 g                                                                                  1.0 g                                     ______________________________________                                    

The compositions were prepared according to the procedure described in Example 3.

The compositions were thermally cycled between 4° C. (24 hours) and 30° C. (24 hours) for a period of one month. Microscopic analyses revealed no detectable mometasone furoate monohydrate crystal growth under these conditions. 

We claim:
 1. 9.alpha.,21-dichloro-16α-methyl-1,4-pregnadiene-11β,17α-diol-3,20-dione-17-(2'-furoate) monohydrate.
 2. A pharmaceutical composition comprising an antiinflammatory amount of mometasone furoate monohydrate in a pharmaceutically acceptable carrier.
 3. The composition of claim 2, further comprising the following ingredients:

    ______________________________________                                         Ingredients          mg/g                                                      ______________________________________                                         Mometasone furoate monohydrate                                                                         0.1-10                                                 Microcrystalline cellulose and sodium                                                                  20                                                     carboxymethyl cellulose                                                        Glycerin                21                                                     Citric acid             2                                                      Sodium citrate          2.8                                                    Polysorbate 80          0.1                                                    Benzalkonium chloride   0.2                                                    Phenylethyl alcohol     2.5                                                    Purified water q.s. ad  1.0     g                                              ______________________________________                                    


4. The composition of claim 3 comprising 0.5 mg of mometasone furoate monohydrate.
 5. The compound 9α,21-dichloro-16α-methyl-1,4-pregnadiene-11β,17α-diol-3,20 dione-17-(2'-furoate) monohydrate exhibiting a x-ray crystallographic powder diffraction pattern having essentially the following values:

    ______________________________________                                         Angle of      Spacing                                                          2θ      d        Relative Intensity                                      (degrees)     (Å)  |/|                                   ______________________________________                                          7.795        11.3324  100                                                     11.595        7.6256   6                                                       12.035        7.3478   3                                                       12.925        6.8437   11                                                      14.070        6.2893   22                                                      14.580        6.0704   5                                                       14.985        5.9072   12                                                      15.225        5.8146   33                                                      15.635        5.6631   96                                                      16.710        5.3011   15                                                      17.515        5.0592   14                                                      18.735        4.7324   12                                                      20.175        4.3978   13                                                      20.355        4.3593   6                                                       20.520        4.3246   4                                                       21.600        3.1108   5                                                       21.985        3.0396   22                                                      22.420        3.9622   8                                                       22.895        3.8811   7                                                       23.245        3.8234   14                                                      23.550        3.7746   13                                                      24.245        3.6680   4                                                       24.795        3.5878   11                                                      24.900        3.5729   5                                                       25.800        3.4503   5                                                       25.985        3.4262   3                                                       26.775        3.3268   84                                                      27.170        3.2794   10                                                      27.305        3.2635   9                                                       27.710        3.2167   5                                                       28.385        3.1417   7                                                       29.165        3.0594   1                                                       29.425        3.0330   2                                                       29.725        3.0030   2                                                       30.095        3.9670   7                                                       30.255        2.9516   3                                                       30.490        2.9294   10                                                      30.725        2.9075   6                                                       31.115        2.8720   3                                                       31.595        2.8294   47                                                      32.135        2.7831   6                                                       32.985        2.7133   7                                                       33.400        2.6805   2                                                       33.820        2.6482   2                                                       34.060        2.6301   8                                                       34.625        2.5885   4                                                       34.795        2.5762   2                                                       35.315        2.5394   1                                                       36.780        2.4416   21                                                      37.295        2.4090   2                                                       ______________________________________                                    


6. A pharmaceutical composition comprising mometasone furoate monohydrate in a carrier consisting essentially of water.
 7. The pharmaceutical composition of claim 6 wherein said mometasone furoate monohydrate is present in an amount of from about 0.1 mg. to about 10.0 mg per gram of water.
 8. The pharmaceutical composition of claim 6 wherein said mometasone furoate monohydrate is present in the form of micronized particles.
 9. The pharmaceutical composition of claim 6 wherein said composition has a pH of from about 4.0 to 5.0.
 10. The pharmaceutical composition of claim 6 further comprising one or more additional components selected from the group consisting essentially of excipients, suspending agents, buffers, surfactants, preservatives and mixtures thereof.
 11. The pharmaceutical composition of claim 6 formulated as a nasal spray.
 12. The pharmaceutical composition of claim 6 wherein said mometasone furoate monohydrate is suspended in said aqueous carrier. 